Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 1. MBP-stimulated CD4 and CD8 expressing PD-1. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, PD-1-expressing MBP-stimulated CD4 T lymphocytes. Bottom panels, PD-1-expressing MBP-stimulated CD8 T lymphocytes. In the upper right corners, the percentage of CD4/PD-1 and CD8/PD-1 T cells relative to the total number of lymphocytes are indicated. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 4. Annexin V- and PD-1-expressing MBP-stimulated CD4 and CD8 T lymphocytes. Representative results of MBP-stimulated PBMC of patients with either AMS or SMS are shown. Top panels, MBP-stimulated CD4 T lymphocytes expressing annexin V and PD-1. Bottom panels, MBP-stimulated CD8 T lymphocytes expressing annexin V and PD-1. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 5. pAkt-expressing MBP-stimulated CD4 and CD8 T lymphocytes. Representative results of MBP-stimulated PBMC of patients with either AMS or stable SMS are shown. Top panels, MBP-stimulated CD4 T lymphocytes expressing pAkt. Bottom panels, MBP-stimulated CD8 T lymphocytes expressing pAkt. Summary results obtained in AMS and SMS patients are presented in A and B. The boxes stretch from the 25th to the 75th percentile; the lines across the boxes indicate the median values; the lines stretching from the boxes indicate extreme values. Statistical significance is shown.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Costimulatory pathways in multiple sclerosis: distinctive expression of PD-1 and PD-L1 in patients with different patterns of disease.

doi: 10.4049/jimmunol.0901038

Figure Lengend Snippet: FIGURE 6. Blockade of the PD-1-PD-L1 pathway. pAKT-expressing, MBP-stimulated CD4 and CD8 T lymphocytes.

Article Snippet: The following mAbs were used in this study: anti-human CD4 (clone 13B8.2; mouse IgG2a isotype), anti-human CD14 (clone 116; mouse IgG1 isotype), anti-human CD19 (clone J4.119; mouse IgG1 isotype) coupled to R-PE-Cyanine 5 Tandem (PE-Cy5; Caltag Laboratories); anti-human CD8 (clone SFCI21thy2D3; mouse IgG1 isotype) PE-Cyanin-7 (BeckmanCoulter); anti-human CD86 (clone BU63; mouse IgG1 isotype), antihuman CD80 (clone 3H5; mouse IgG1 isotype; Serotec) coupled to FITC; anti-human PD-L1 (gift from Dr. L. Chen) and anti-mouse IgG (H L chain) coupled to FITC (eBioscience); anti-human-B7H3 (gift from Dr. L. Chen) coupled FITC anti-human PD-1 (clone MIH4) coupled to PE (mouse-IgG1 isotype; eBioscience); and anti-human annexin V coupled to FITC (mouse-IgG1 isotype; Beckman Coulter).

Techniques: Expressing

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Armored Inducible Expression of IL-12 Enhances Antitumor Activity of Glypican-3-Targeted Chimeric Antigen Receptor-Engineered T Cells in Hepatocellular Carcinoma.

doi: 10.4049/jimmunol.1800033

Figure Lengend Snippet: FIGURE 6. GPC3-m28Z-mNFAT-mIL-12 T cells improved antitumor immunity against established murine HCC tumor. (A) Left panel, Experimental scheme of in vivo immunocompetent model. Murine T cells at different doses were transferred i.v. into female C57BL/6 mice 8 d after tumor cell in- oculation (n = 6). Right panel, Tumor growth curve for Hepa1-6-GPC3 xenografts treated with indicated murine T cells. Arrow indicates murine T cells infusion. (B) The tumor weight was measured at the study end point. (C) Body weight of each group was measured every 3–4 d (baseline = 100%). (D) The amounts of mIFN-g and mIL-12 (p70) in the sera of treated mice 8 d after therapy were assessed by ELISA. Data shown are the mean 6 SEM. (E) CAR copy numbers in genomic DNA of residual tumors 8 d after therapy were measured by real-time PCR. (F) The sections of formalin-fixed, paraffin- embedded tumor tissue were immunostained with anti-mouse CD8a Ab or anti-mouse Foxp3 Ab. The images were obtained under original magnification 3400. Data shown are from three independent experiments. *p , 0.05, **p , 0.01, ***p , 0.001.

Article Snippet: To detect murine CD8+ T cells and regulatory T cells (Tregs), the sections of formalin-fixed, paraffin-embedded tumor tissue were immunostained with anti-mouse CD8a Ab (Cell Signaling Technology) or anti-mouse Foxp3 mAb (eBioscience), followed by goat anti-rat IgG-HRP (Santa Cruz Biotechnology) or HRP-conjugated rabbit anti-mouse IgG (H&L) (KangChen Biotech).

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

Journal: Frontiers in Immunology

Article Title: Inhibition of PCSK9 enhances the antitumor effect of PD-1 inhibitor in colorectal cancer by promoting the infiltration of CD8 + T cells and the exclusion of Treg cells

doi: 10.3389/fimmu.2022.947756

Figure Lengend Snippet: In vivo antitumor effect of anti-PD-1 antibody. (A) Tumor volume and tumor weight in the MC38 tumor model. Anti-PD-1 mAb administration was 5 mg/kg, n = 4 mice/group. (B) Tumor volume and tumor weight of CT26 tumor model. Anti-PD-1 mAb administration was 5 mg/kg, n = 3 mice/group. (C, D) Flow cytometry analysis of tumor-infiltrating T cells for mice treated with PBS or anti-PD-1 antibody in MC38 tumor model (C) and in CT26 tumor model (D) . (E, F) IHC staining of CD8a and Foxp3 in CT26 tumors. "*" means p-value < 0.05 and "**" means p-value < 0.01.

Article Snippet: The following were the antibodies used: rabbit anti-mouse CD8a antibody (Servicebio, GB11068), rabbit anti-mouse Foxp3 antibody (Servicebio, GB112325), rabbit anti-mouse CSF1R antibody (Servicebio, GB11581), rabbit anti-mouse NK1.1 antibody (abcam, ab289542), rabbit anti-mouse CD11b antibody (Servicebio, GB11581), and rabbit anti-mouse CD4 antibody (Servicebio, GB13064-2).

Techniques: In Vivo, Flow Cytometry, Immunohistochemistry

Journal: Frontiers in Immunology

Article Title: Inhibition of PCSK9 enhances the antitumor effect of PD-1 inhibitor in colorectal cancer by promoting the infiltration of CD8 + T cells and the exclusion of Treg cells

doi: 10.3389/fimmu.2022.947756

Figure Lengend Snippet: (A) IHC staining of CD8a in tumor of the MC38 tumor model treated with anti-PD-1 or anti-PCSK9 antibody and quantitative analysis of positive particles and (B) for the CT26 tumor model. (C) Flow cytometry analysis of CD45 + , CD3 + , and CD8 + T-cell infiltration in MC38 tumors. "*" means p-value < 0.05 and "**" means p-value < 0.01.

Article Snippet: The following were the antibodies used: rabbit anti-mouse CD8a antibody (Servicebio, GB11068), rabbit anti-mouse Foxp3 antibody (Servicebio, GB112325), rabbit anti-mouse CSF1R antibody (Servicebio, GB11581), rabbit anti-mouse NK1.1 antibody (abcam, ab289542), rabbit anti-mouse CD11b antibody (Servicebio, GB11581), and rabbit anti-mouse CD4 antibody (Servicebio, GB13064-2).

Techniques: Immunohistochemistry, Flow Cytometry

Journal: Oncology Letters

Article Title: Survival analysis with regard to PD-L1 and CD155 expression in human small cell lung cancer and a comparison with associated receptors

doi: 10.3892/ol.2019.9910

Figure Lengend Snippet: Immunofluorescence double staining of PD-1/TIGIT and CD8 in small cell lung cancer. Nuclear staining with DAPI (blue); CD8 staining with TRITC-goat anti-rabbit second antibody (red) or FITC-donkey anti-rabbit second antibody (green); PD-1 staining with FITC-goat anti-mouse second antibody (green); TIGIT staining with TRITC-donkey anti-goat second antibody (red). Sections were photographed at magnification, ×400. CD, cluster of differentiation; PD, programmed death; TIGIT, T cell immunoreceptor with immunoglobulin and ITIM domains; FITC, fluorescein isothiocyanate; TRITC, tetramethylrhodamine; TILs, tumor-infiltrating lymphocytes.

Article Snippet: Sections were incubated with primary anti-TIGIT antibody and anti-CD8 antibody (1:75 dilution; cat. no. 17335-1-AP; ProteinTech Group, Inc.), or with anti-PD-1 antibody and anti-CD8 antibody (1:75 dilution; cat. no. 17335-1-AP; ProteinTech Group, Inc.) at 4°C.

Techniques: Immunofluorescence, Double Staining, Staining

Journal: Molecular therapy : the journal of the American Society of Gene Therapy

Article Title: Oncolytic adenovirus in treating malignant ascites: A phase II trial and longitudinal single-cell study.

doi: 10.1016/j.ymthe.2024.04.029

Figure Lengend Snippet: Figure 4. Characteristics and dynamics of CD8+ and CD4+ T cell subsets during H101 treatment (A) Distinct cell subtypes of CD8+ T cells demonstrated by UMAP plot. (B) Cell proportion of each CD8+ T cell subtype. Black asterisks represent the statistical significantly increased proportion on day 7 compared with that at baseline. (C) Fraction of CD8_MKI67 cells in two response groups on days 0, 7, and 14. Wilcoxon test. *p < 0.05. (D) Representative double-color immunofluorescence staining of CD8+Ki67+ cells in MA samples (P04 [T-long patient] and P07 [T-short patient]) on days 0, 7, and 14, with

Article Snippet: For double-color immunofluorescence of CD8A and Ki67, mouse anti-human CD8a (CST, Cat#70306) and rabbit anti-human Ki67 (Abcam, Cat#15580), were applied.

Techniques: Staining

Journal: Research in Veterinary Science

Article Title: Relationship between rate of infection and markers of inflammation/immunity in Holy Birman cats with feline coronavirus

doi: 10.1016/j.rvsc.2014.08.009

Figure Lengend Snippet: Results recorded in Birman cats and in cats from other breeds.

Article Snippet: Immunophenotyping by flow cytometry was performed on aliquots of 50 μL of the cell suspension to identify lymphocyte subpopulations as previously described ( ) using the following panel of antibodies for feline surface antigen: 2.5 μL of mouse anti feline CD4 (specific for T helper cells, clone MCA1350, Serotec, Oxford, UK), 1 μL of mouse anti feline CD8 alpha/beta (specific for T cytotoxic cells, clone MCA1347G, Serotec, Oxford, UK), 50 μL of mouse anti feline CD5 (specific for T cells, clone MCA2038S, Serotec, Oxford, UK), and 1 μL of mouse anti canine CD21, specific for B cells, that cross reacts with feline species (clone MCA1781R, Serotec).

Techniques: